Cheng, C-Y., Krishnakumar, V., Chan, A. P., Thibaud-Nissen, F., Schobel, S., Town, C. D. (2016). Araport11: a complete reannotation of the Arabidopsis thaliana reference genome. Plant J, Accepted Author Manuscript.
The flowering plant Arabidopsis thaliana is a dicot model organism for research in many aspects of plant biology. A comprehensive annotation of its genome paves the way for understanding the functions and activities of all types of transcripts, including mRNA, the various classes of non-coding RNA, and small RNA. The TAIR10 annotation update had a profound impact on Arabidopsis research but was released more than five years ago. Maintaining the accuracy of the annotation continues to be a prerequisite for future progress. Using an integrative annotation pipeline, we assembled tissue-specific RNA-Seq libraries from 113 datasets and constructed 48,359 transcript models of protein-coding genes in eleven tissues. In addition, we annotated various classes of non-coding RNA including microRNA, long intergenic RNA, small nucleolar RNA, natural antisense transcript, small nuclear RNA, and small RNA using published datasets and in-house analytic results. Altogether, we identified 635 novel protein-coding genes, 508 novel transcribed regions, 5,178 non-coding RNAs, and 35,846 small RNA loci that were formerly unannotated. Analysis of the splicing events and RNA-Seq based expression profiles revealed the landscapes of gene structures, untranslated regions, and splicing activities to be more intricate than previously appreciated. Furthermore, we present 692 uniformly expressed housekeeping genes, 43% of whose human orthologs are also housekeeping genes. This updated Arabidopsis genome annotation with a substantially increased resolution of gene models will not only further our understanding of the biological processes of this plant model but also of other species.
Krishnakumar, V., Hanlon, M. R., Contrino, S., Ferlanti, E. S., Karamycheva, S., Kim, M., Rosen, B. D., Cheng, C., Moreira, W., Mock, S. A., Stubbs, J., Sullivan, J. M., Krampis, K., Miller, J. R., Micklem, G., Vaughn, M. & Town, C. D. (2014). Araport: the Arabidopsis Information Portal. Nucl. Acids Res., 43(D1), D1003-D1009.
The Arabidopsis Information Portal (https://www.araport.org) is a new online resource for plant biology research. It houses the Arabidopsis thaliana genome sequence and associated annotation. It was conceived as a framework that allows the research community to develop and release ‘modules’ that integrate, analyze and visualize Arabidopsis data that may reside at remote sites. The current implementation provides an indexed database of core genomic information. These data are made available through feature-rich web applications that provide search, data mining, and genome browser functionality, and also by bulk download and web services. Araport uses software from the InterMine and JBrowse projects to expose curated data from TAIR, GO, BAR, EBI, UniProt, PubMed and EPIC CoGe. The site also hosts ‘science apps,’ developed as prototypes for community modules that use dynamic web pages to present data obtained on-demand from third-party servers via RESTful web services. Designed for sustainability, the Arabidopsis Information Portal strategy exploits existing scientific computing infrastructure, adopts a practical mixture of data integration technologies and encourages collaborative enhancement of the resource by its user community.
Hanlon, M. R., Vaughn, M., Mock, S., Dooley, R., Moreira, W., Stubbs, J., Town, C., Miller, J., Krishnakumar, V., Ferlanti, E., and Pence, E. (2015). Araport: an application platform for data discovery. Concurrency Computat.: Pract. Exper., doi: 10.1002/cpe.3542.
Araport is an open-source, online community resource for research on the Arabidopsis thaliana genome and related data. Araport is developed through a partnership between J. Craig Venter Institute, the Texas Advanced Computing Center at The University of Texas at Austin, and The University of Cambridge. Part of the open architecture of Araport is the Science Applications Workspace. Taking an ‘app store’ approach, users can choose applications developed both by the Araport team and community developers to create a customized environment for their work. Araport also provides tooling and support for developing applications for Araport, including an application generator, a rapid development and testing tool, and a straightforward deployment path for publishing applications into the Araport workspace.
International Arabidopsis Informatics Consortium. (2012). Taking the next step: building an Arabidopsis information portal. The Plant Cell, 24(6), 2248-2256.
The Arabidopsis Information Portal (AIP), a resource expected to provide access to all community data and combine outputs into a single user-friendly interface, has emerged from community discussions over the last 23 months. These discussions began during two closely linked workshops in early 2010 that established the International Arabidopsis Informatics Consortium (IAIC). The design of the AIP will provide core functionality while remaining flexible to encourage multiple contributors and constant innovation. An IAIC-hosted Design Workshop in December 2011 proposed a structure for the AIP to provide a framework for the minimal components of a functional community portal while retaining flexibility to rapidly extend the resource to other species. We now invite broader participation in the AIP development process so that the resource can be implemented in a timely manner.
|Can I advertise a conference/event on Araport?|
Staff at Araport would be happy to post a ad for you. Please send us the title, URL (if available), date, and a brief description of the event. We will promptly post it to the 'Conferences and Meetings' board.
|Can I post a job ad on Araport?|
Yes, you can post jobs on Araport and it is free of charge. Please register, log in, visit the job page, and click on the "post a job" button.
Alternatively, we could also post it for you if you prefer. Please send the details about the position or a flyer with the information to firstname.lastname@example.org
|How do I link to Araport?|
We welcome links to Araport.
To access a free-text search of all... READ MORE ...
|I still have questions. What should I do?|
|Who maintains the Araport website?|
Araport is the web site of the Arabidopsis Information Portal project. Araport is maintained by scientists at the J. Craig Venter Institute (JCVI), the Texas Advanced Computing Center (TACC) at University of Texas Austin, Cambridge System Biology Centre (CSBC), and other collaborators. Araport is supported by grants from the National Science Foundation (NSF) in the USA and the BBSRC in the UK. The Araport web site intended to foster scientific research and... READ MORE ...